Heparinase High Purity Enzymes for Glycobiology Research
IBEX has developed a proprietary expression system for the production of our enzymes in Flavobacterium heparinum. This system allows for the production of each glycosaminoglycan enzyme in a way that minimizes the cross contamination of other GAG enzymes which can occur when enzymes are produced from the native strain of Flavobacterium heparinum (Pedobacter heparinus). When combined with our well-defined proprietary purification process our enzymes are of the highest purity.
Due to its high purity and reaction time IBEX Heparinase I is the only heparinase used in FDA and EU approved hemostasis-measuring Point Of Care devices.
| Product | Substrate | Purity* |
| Heparinase I | Heparin & Heparan Sulfate | ≥ 95% |
| Heparinase II | Heparan Sulfate & Heparin | ≥ 90% |
| Heparinase III | Heparan sulfate | ≥ 95% |
| * By reversed phase HPLC analysis | ||
| Free shipping on lyophilized vials | ||
| The IBEX Quality Management System is Certified ISO 13485 for the Manufacturing of Enzymes sold as reagents or incorporated in diagnostic devices. | ||
Heparinase I
Synonyms: Heparinase; heparin lyase; heparin eliminase
Source: Flavobacterium heparinum (recombinant)
Catalyzed Reaction: The enzyme cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and Osulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.
Substrate Specificity: Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate).
Heparinase II
Source: Flavobacterium heparinum (recombinant)
Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharidechains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.
Substrate Specificity: Heparin, heparan sulfate.
Heparinase III
Source: Flavobacterium heparinum (recombinant)
Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.
Substrate Specificity: Heparin, heparan sulfate.
| Heparinase I | Heparinase II | Heparinase III |
|---|---|---|
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IBEX produces high purity enzyme products that do not contain preservative agents or proteinaceous stabilizers such as albumins.
Our enzymes are sold in either lyophilized or frozen liquid form. Enzymes formulated as liquids are shipped on dry ice. Each shipment is accompanied by a certificate of analysis.
IBEX sells its enzymes in International Units (IU). One international unit is defined as “the amount of enzyme that will liberate 1.0 µmole per minute of unsaturated oligosaccharides from substrates at 30°C and pH 7.0 (Heparinase I), 7.5 (Heparinase II and III)”.